بحث منشور للدكتور وصفي ظاهر بعنوان THE EFFECT OF HCG, 17 Β-ESTRADIOL AND HYDROCORTISONE ON BLOOD CHOLESTEROL

THE EFFECT OF HCG, 17 Β-ESTRADIOL AND HYDROCORTISONE ON BLOOD CHOLESTEROL, HDL,LDL TG ,TOTAL PROTEIN, GLUCOSE ,PH AND BODY WEIGHT IN FEMALE RABBITS. Wasfi Dhahir Abd Ali Al -Mahmod Department of physiology ,Collage of Veterinary Medicine , University of Basrah,Basrah,Iraq Key words: HCG, 17 β- estradiol , cholesterol (Received 31 March 2009, Accepted 28 April 2009) ABSTRACT The aim of this study is to investigate the effect of human chorionic gonadotophic (HCG), 17β-estradiol and hydrocortisone on some blood parameters and body weight in female rabbits. Twenty local rabbits were subdivided randomly and equally into four groups . The1st group used as control . The 2nd group was injected daily (30 IU /kg B.W) HCG intramuscularly . The 3rd group was injected subcutaneously daily with 0.1 mg /kg B.W s/c 17 β-estradiol and the 4th group was injected daily 0.1 mg /kg B.W intramuscularly hydrocortisone. After five weeks blood serum was tested for total Cholesterol, HDL(height density lipo-protein), LDL (low density lipo- protein),TG (triglycerides), total protein and pH. Body weight was taken dialy from first day . The results showed that the administration of HCG raised high significantly LDL(p>0.01) while total Cholesterol , TG, glucose failed to reach the significant level, .However HCG significantly lowered HDL (p>0.01) Total protein and body weight were decreased with no change in blood pH compared with the control group. The treatment with 17β-estradiol decreased the level of cholesterol , , TG and lowered significantly HDL(p 0.05 > and increased significantly LDL (p > ( 0.05. Glucose and total protein were increased with no significant effect on body weight and pH compared with the control group. While hydrocortisone increased ,HDL ,TG ,LDL and glucose and decreased cholesterol and total protein level moreover body weight and pH did n,t effected. INTRODUCTION Hormones are chemical compounds which have biological activities and play an important role in metabolic process (1). It had been shown that 17β-estradiol decreased cholesterol and lower Triglycerides (TG) (2). (3) showed that after oral administration of higher dose of estrogen total cholesterol decreased significantly. Another study showed that 17β- estradiol lowered glucose because of raising insulin level(4) . However anther study found that 17β- Estradiol inhibited the insulin and increased glucose(5). (6) Investigated that contraceptive which contain estradiol decreased HDL and LDL. While on other Investigation found that estradiol increased HDL and TG Bas.J.Vet.Res.Vol.8,No.1,2009. significantly decreased (7) . (8) found that 17β-estradiol increased HDL and cholesterol level and decreased LDL and total protein . About the effect of estradiol on body weight a study pointed that estradiol increased the body weight (9). (10) found that HCG enhanced HDL-cholesterol transport. Another study found that HCG increased blood glucose (18.78%) (11). (12) found HCG increased cholesterol but estradiol decreased it level and gave evidence that estradiol and hydrocortisone have similar effect on serum glucose, HDL, LDL and all of them increased serum glucose ,LDL, HDL and total protein , but show different effect on triglycerides. A study found that pregnant gilts treated with hydrocortisone caused an increased in total blood serum cholesterol, triglycerides and total protein(13). Thus the main purpose of the study is to investigate the different effects of HCG, estradiol and hydrocortisone on some blood parameters as comparative study between them to ovoid the risk of blood parameters alteration. MATERIAL AND METHODS Twenty female rabbits from local markets weighted 500-700 gram were divided equally and randomly into four groups , 1st group used as control 2nd group were injected daily HCG (30 iu /kg B.W) i/m. (MC-Chypre company ), the 3rd group were injected daily with 0.1 mg /kg B.W s/c β-17estradiol (Syva .lab company) and the 4th group were injected daily 0.1 mg /kg B.W i/m hydrocortisone(Alvertran Werfft AG-Viena company) Animal weighed daily till the end of treatment, after five weeks animals were anaesthetized by inhalation of Diethyl ether and rapidly dissected and blood sample were collected from the abdominal vein(vena cava) in glass centrifuge tubes then centrifuged for 15 min at 1000 g ,Sera were separated and tested for total Cholesterol, HDL,LDL,TG total protein and glucose concentration were determined automatically using auto analyzer,( Randox kits ( SLIM 260 -Ascreen Master produced by SEAC, while the pH of blood serum measured by pH strips. Data were represented as means + S .D , and percentage differences; the statistical analysis by Student- t -test (14). RESULTS The administration of HCG to female rabbits for 5 weeks raised the level of blood serum Cholesterol (49.25%), TG(120.75%)(table1) and glucose(18.78%),table(2),and high significant increased in LDL (92.5%)(p>0.01) table(1), and significant decreased in the level of HDL(-35.48%) (p>0.01) table (1), and non significant decreased in total protein (-18.90%) in serum. Body weight were decreased (-14.88) but blood pH was not effected .Table (3) . Bas.J.Vet.Res.Vol.8,No.1,2009. The study show that 17β-estradiol decreased the level of cholesterol (-47.53%), TG(-43.34) (table1) and caused significant decreased in HDL(-33.33%)(p > 0.05) and increased the level of LDL(22.24%) (p > 0.05) table( 1). glucose(17.58%) and total protein (20.13%)(table2) with no significant effects on body weight and pH . Table (3) . Hydrocortisone Caused increased in the level of LDL (58.35%) ,TG(12.35) (table1) and glucose (26.34%)(table2) and decreased the cholesterol (-14.98%)(table1) and Total protein(-12.3%) with no effect on blood pH and body weight. Table( 3). Table (1) :The effects of HCG, 17 β-estradiol, and hydrocortisone on cholesterol ,LDL HDL and TG. n=5 Hydrocortisone estradiol β- 17 DISCUSSION Hormones of the resent study (HCG, 17β- estradiol, and hydrocortisone) was demonstrate changes in some blood parameters. The results in table (1) show that HCG increased cholesterol(49.25%), TG(2075%) and LDL (92.5%) and cause significant decreased in HDL (-35.48%). (10) pointed that HDL was enhanced by HCG. (15) pointed that HCG have effects on fatty acids synthesis and may be lead to form new triglycerides.(11) reported that treatment with Hydrocortisone estradiol β- 17 HCG Control Diff % Mean SD + Diff % Mean SD + Diff HCG increased blood glucose and profoundly inhibited the development of diabetes this insure our result (18.78%). Table(3) showed that HCG decreased body weight (-14.88). A study found that women whom used HCG lost (20lb) of their weight within one month(16). Table (1) showed that administration of 17β- estradiol caused decreased in percentage differences of cholesterol (-47-53%), TG (-43.43%) and a significant increased in HDL (-33.33%), and LDL (22.24%) (p>0.05). A study found that estradiol increased HDL and TG(17).Another study found that estradiol activated the transformation of cholesterol to other hormones and this cause decreased in it in blood serum(18). As well as another investigated that 17β -estradiol lowered the level of cholesterol and TG(19). While another researchers found that estradiol decreased cholesterol. and increased LDL and HDL, with no change in TG(20).And (6) investigated that contraceptives which contain estradiol caused decreased in HDL. Another study concluded that HDL increased at 3rd month and TG decreased(21). In another study found that estradiol reduced total cholesterol and LDL (22), while this study showed that 17β- estradiol caused decreased in total serum cholesterol but increased significantly LDL (22.24%) (p>0.05). (7)showed that estradiol increased HDL and higher significant in serum total cholesterol and TG. Estradiol caused increased in percentage differences in total protein (20.13%) and glucose (17.58%)( Table (2).An investigations found that 17β - estradiol lower the level of glucose because of raising insulin (4) While another study pointed that estradiol inhibit the insulin and increased glucose level (5). Estrogen have effect on carbohydrate metabolism and it might be therefore be expected that their use would result in corresponding increased glucose and the incidence of diabetes(23).While the resent study show that glucose decreased with no effect on HDL,LDL and TG after estrogen therapy(24). Hydrocortisone decreased serum total cholesterol (-14.94%) and increased in TG (12.3%) .(25) concluded that the treatment with hydrocortisone increased the level of cholesterol and TG. Hydrocortisone increased serum glucose(26.32%).A study pointed that the decrease in corticosteroids lead to lowering blood glucose level (26). LDL was increased after administration of hydrocortisone (58.35%).(27) found that hydrocortisone increased plasma level of LDL due to reducing hepatic LDL receptors. The results in Table (2) showed 17B-estradiol , HCG and Hydrocortisone had no significant effects on blood pH. 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